Research

Our current work

Theme 01

Building a functional map of cell surface glycans

Using functional genomics and glycoengineering, we showed that glycans are a key mediator of immune evasion in acute myeloid leukemia. O-glycosylation and sialylation, particularly of the glycoprotein CD43, forms a steric shield that physically restrains immune cell interactions.

We are now asking how glycans remodel the architecture of the cell surface, and how their dysregulation alters cell function in diseases like cancer.

Theme 02

Decoding how immune cells sense and respond to cell surface glycans

More than 100 immune receptors have evolved to recognize glycans as ligands, making glycans powerful immunomodulatory motifs that transmit signals both biophysically and biochemically.

We investigate how glycans shape immune signaling and organize the immune synapse, using functional genomics and proteomics to interrogate intercellular interactions at scale in primary immune cells.

Theme 03

Engineering next-generation immune therapies

Cell surface glycans are abundant, tightly regulated, immune-evasive, and often specific to a given tumor or cell type. They also form a biophysical barrier that impedes both immune cell interactions and antibody binding.

Together these properties make the glycocalyx an especially attractive therapeutic target. We are building on our work to develop glycoform-selective antibodies as well as antibody and engineered cell therapies that remodel the cancer glycocalyx.

Toolkit

Our Approach

We integrate genetic and proteomics strategies to map and dissect the function of cell surface glycosylation in intercellular interactions, with applications for cancer.

Functional genomics

Genome-wide and intercellular CRISPR screens, using primary human cells to discover intercellular interactions.

Proximity proteomics

Proximity-induced labeling to resolve surface neighborhoods at nanometer scale.

Glycoengineering

Cells with defined, engineered glycosylation states to study the phenotypic implications of glycan dysregulation.

Super-resolution imaging

Live-cell and super-resolution microscopy to study cell surface topology and immune synapse formation.

Primary human models

Primary human models including hematopoietic stem cells, monocyte-derived and iPSC-derived macrophages, and patient samples, used for screens and to validate our hypotheses.

Computational biology

Integrative analysis across CRISPR screens, proteomics, transcriptomics, and imaging datasets to model and define the function of cell surface glycans.

Interested in learning more?